Via Slavica is joined from the Czech republic second biggest city Brno. The cycling trail alone from Brno via Židlochovice, Pasohlávky and Nový Přerov to border crossing Hevlín/Laa an der Thaya and further on via Mistelbach and Wolkersdorf to Vienna is more than 170 km long. The entire route in the South Moravia region is signposted with standard Czech signs (yellow signs) with the Cycle trail Brno – Vienna logo. It is also numbered as national cycle route 5. Cycle trail Brno – Vienna.